The classic comprehensive and authoritative textbook on bacterial molecular genetics. Features completely revised and updated material and new chapters, incorporating the most recent advances in the field since publication of the third edition in 2007. • Provides thought questions, problems, and suggested reading lists for each chapter that test student comprehension and encourage further research. • Provides descriptive background information, detailed experimental methods, examples of genetic analyses, and advanced material relevant to current applications of molecular genetics. • Serves as an invaluable text for anyone working in the fields of microbiology, genetics, biochemistry, bioengineering, medicine, molecular biology, and biotechnology. It is also essential reading for scientists in all fields of biology, many of whom depend upon the concepts and techniques covered in this book.